Immunohistochemistry (Frozen sections) abreview for Anti-Vimentin antibody [RV202] - Cytoskeleton Marker

Immunohistochemistry (Frozen sections)

Rat Cell (ED18 embryo)

Yes - 0.3% TritonX in 0.1% PBS

ED18 embryo

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Paraformaldehyde

24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% TritonX with 0.1x PBS- 10% Donkeys

1/1000

1/1000

Non-Abcam antibody was used: Alexa Fluro 568
Host species: Mouse
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568

IHC-Fr image of Ed18 rat stained with ab8978. Fresh frozen sections were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3% triton X100 for 1h to permeabilise the tissues and block non-specific protein-protein interactions. The sectons were then incubated with the antibody (ab8978)., 1µg/ml) and (ferroportin) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) donkey anti-mouse at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Vimentin expressed in the gut muscles.